Methylglyoxal Comes of AGE

Iranian Journal of Basic Medical Sciences comes of age on the 20th anniversary of publication

Isolation and identification of 5-methyl-imidazolin-4-one derivative as glyceraldehyde-derived advanced glycation end product.

We isolated and identified the glyceraldehyde-derived advanced glycation product (AGE) formed from glyceraldehyde and N(alpha)-acetylarginine. A major product was identified as N(alpha)-acetyl-N(delta)-(5-methyl-imidazolin-4-one-2-yl)-ornithine. The compound has been reported as methylglyoxal-derived AGE, MG-H1. This study suggests that MG-H1 is formed through both glyceraldehyde-related and me...

Study on the changes of carbonic anhydrase activity in insulin resistance and the effect of methylglyoxal.

OBJECTIVES To determine the serum levels of methylglyoxal (MG) and carbonic anhydrase (CA) activity in known cases of type-II diabetes mellitus with insulin resistance and to compare them with age-sex matched healthy controls. METHODS Eightysix participants were enrolled for the present case-controlled study with ages ranging from 25 to 44 years. The analytes measured were fasting blood gluco...

Effects of Methylglyoxal and Aspirin on In Vitro Coagulation and Clot Permeability

Backgrounds and Aims: Methylglyoxal (MGO) is an –α, β dicarbonyl aldehyde inevitably produced from triose-phosphate intermediates of glycolysis, and amino acid. Increased MGO in blood leads to alterations in coagulation, clot permeability and thus, atherosclerosis in children with diabetes; however, the precise mechanism is not clear. The present study aimed to compare differen...

Methylglyoxal-derived hydroimidazolone advanced glycation end-products of human lens proteins.

PURPOSE To determine the concentrations of methylglyoxal-derived advanced glycation end-products (AGEs), the hydroimidazolones MG-H1 and -H2, in soluble human lens proteins and compare them with the concentrations of other methylglyoxal-derived AGEs and pentosidine. METHODS Lens protein samples were hydrolyzed enzymatically. AGEs were assayed without derivatization by HPLC with tandem mass sp...